Spectroscopic Estimation of Biomass in Canopy Components of Paddy Rice Using Dry Matter and Chlorophyll Indices

Crop biomass is a critical variable for characterizing crop growth development, understanding dry matter partitioning, and predicting grain yield. Previous studies on the spectroscopic estimation of crop biomass focused on the use of various spectral indices based on chlorophyll absorption features and found that they often became saturated at high biomass levels. Given that crop biomass is commonly expressed as the dry weight of canopy components per unit ground area, it may be better estimated using the spectral indices that directly characterize dry matter absorption. This study aims to evaluate a group of four dry matter indices (DMIs) by comparison with a group of four chlorophyll indices (CIs) for estimating the biomass of individual components (e.g., leaves, stems) and their combinations with the field data collected from a two-year rice cultivation experiment. The Red-edge Chlorophyll Index (CIRed-edge) of the CI group exhibited the best relationship with leaf biomass (R2 = 0.82) for the whole growing season and with total biomass (R2 = 0.81), but only for the growth stages before heading. However, the Normalized Difference Index for Leaf Mass per Area (NDLMA) of the DMI group showed the best relationships with both stem biomass (R2 = 0.81) and total biomass (R2 = 0.81) for the whole season. This research demonstrated the suitability of dry matter indices and provided physical explanations for the superior performance of dry matter indices over chlorophyll indices for the estimation of whole-season total biomass.